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Student Learning Outcomes

• Students will be able to discuss and demonstrate the ability to perform the maintenance and quality control of equipment used in a histotechnology lab, including tissue processors, embedding centers, microtomes, flotation baths, and ovens
• Students will be able to analyze and troubleshoot H&E and microorganism staining procedures.
• Students will be able to perform coverslipping procedures correctly and efficiently.
• Students will be able to demonstrate the ability to perform hematoxylin and eosin staining and microorganism staining of routine paraffin embedded tissue sections.
• Students will be able to identify the purpose, principle, reagents, function, and action of histological stains for routine H&E staining and selected microorganism staining and the criteria for choosing the appropriate control tissue.
• Students will be able to dismantle and reassemble the knife holder and specimen holder on a rotary microtome.
• Students will be able to identify the purpose, ingredients, function, and action of histological fixatives and the criteria for choosing the appropriate fixative.
• Students will be able to determine acceptable H&E tissue sections.
• Compare and contrast various hematoxylin stains.
• Students will be able to demonstrate the ability to cut acceptable sections while performing routine microtomy of paraffin embedded tissue.
• Students will be able to demonstrate proper orientation and embedding of tissue specimens in paraffin.
• Students will be able to describe and perform tissue processing, including dehydration, clearing, and infiltration.